This is the PDF eBook version for Structural Genomics and Drug Discovery – Methods and Protocols by Wayne F. Anderson
Table of Contents
1. Data Management in the Modern Structural Biology and Biomedical Research Environment
Matthew D. Zimmerman, Marek Grabowski, Marcin J. Domagalski, Elizabeth M. MacLean, Maksymilian Chruszcz, and Wladek Minor
2. Structural Genomics of Human Proteins
Khan Tanjid Osman and Aled Edwards
3. Target Selection for Structural Genomics of Infectious Diseases
Corin Yeats, Benoit H. Dessailly, Elizabeth M. Glass, Daved H. Fremont, Christine A. Orengo
4. Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery
Isabelle Q.H. Phan, Robin Stacy, and Peter J. Myler
5. High Throughput Cloning for Biophysical Applications
Keehwan Kwon and Scott N. Peterson
6. Expression and Solubility Testing in a High Throughput Environment
Keehwan Kwon and Scott N. Peterson
7. Protein Production for Structural Genomics Using E. coli Expression
Magdalena Makowska-Grzyska, Youngchang Kim,Natalia Maltseva, Hui Li, Min Zhou,Grazyna Joachimiak, Gyorgy Babnigg, and Andrzej Joachimiak
8. Eukaryotic Expression Systems for Structural Studies
Christopher A. Nelson, William H. McCoy, and Daved H. Fremont
9. Automated Cell-free Protein Production Methods for Structural Studies
Emily T. Beebe, Shin-ichi Makino, John L. Markley, and Brian G. Fox
10. Parallel Protein Purification
Ludmilla Shuvalova
11. Oxidative Refolding from Inclusion Bodies
Christopher A. Nelson, Chung A. Lee, and Daved H. Fremont
12. High throughput Crystallization Screening
T. Skarina, X. Xu, E. Evdokimova, A. Savchenko
13. Screening Proteins for NMR Suitability
Adelinda A. Yee, Anthony Semesi, Maite Garcia, and Cheryl H. Arrowsmith
14. Salvage or Recovery of Failed Targets by in situ Proteolysis
Yufeng Tong, Aiping Dong, Xiaohui Xu, and Amy Wernimont
15. Salvage of Failed Protein Targets by Reductive Alkylation
Kemin Tan, Youngchang Kim, Catherine Hatzos-Skintges, Changsoo Chang, Marianne Cuff, Gekleng Chhor, Jerzy Osipiuk, Karolina Michalska, Boguslaw Nocek, Hao An, Gyorgy Babnigg, Lance Bigelow, Grazyna Joachimiak, Hui Li, Jamey Mack, Magdalena M. Makowska-Grzyska, Natalia Maltseva, Rory Mulligan, Christine Tesar, Min Zhou, and Andrzej Joachimiak
16. Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy
Lukasz Goldschmidt, David Eisenberg, and Zygmunt S. Derewenda
17. Data Collection for Crystallographic Structure Determination
Kanagalaghatta Rajashankar and Zbigniew Dauter
18. Structure, Determination, Refinement, and Validation
George Minasov and Wayne F. Anderson
19. Virtual High-Throughput Ligand Screening
T. Andrew Binkowski, Wei Jiang, Benoit Roux, Wayne Anderson, and Andrzej Joachimiak
20. Ligand Screening using Fluorescence Thermal Shift Analysis (FTS)
Chi-Hao Luan, Samuel H. Light, Sara F. Dunne, and Wayne F. Anderson
21. Ligand Screening using Enzymatic Assays
Kiira Ratia, Shahila Mehboob, and Hyun Lee
22. Ligand Screening using NMR
Benjamin E. Ramirez, Aleksandar Antanasijevic, and Michael Caffrey
23. Screening Ligands by X-ray Crystallography
Douglas R. Davies
24. Case Study – Structural Genomics and Human Protein Kinases
Jonathan M. Elkins