This is the PDF eBook version for Synthetic mRNA – Production, Introduction Into Cells, and Physiological Consequences by Robert E. Rhoads
Table of Contents
Part I Introduction
1. Synthetic mRNA: Production,
Introduction into Cells, and Physiological Consequences
Robert E. Rhoads
Part
II Synthesis of mRNA
2.
Synthetic
Capped mRNAs for Cap-Specific Photo-Crosslinking Experiments
Joanna Kowalska, Franck Martin, and Jacek Jemielity
3. Enzymatic Modification of 5’-Capped
RNA and Subsequent Labeling by Click Chemistry
Josephin M. Holstein, Daniela Stummer, and
Andrea Rentmeister
4. Preparation of Functional,
Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG
Artem V. Domashevskiy, David J.
Rodriguez, Dilantha Gunawardana, and Dixie J. Goss
5. Intronless b-Globin
Reporter: A Tool for Studying Nuclear
RNA Stability Elements
Jessica A. Brown and Joan A. Steitz
6.
Synthetic mRNA with Superior Properties that Mimics the Intracellular Fates of Natural Histone mRNA
Wei Su, Michael K. Slevin, William F. Marzluff, and Robert E.
Rhoads
7. Engineering WT1-Encoding mRNA to
Increase Translational Efficiency in Dendritic Cells
Daphné Benteyn, Carlo Heirman, Kris
Thielemans, and Aude Bonehill
Part
III Introduction of Synthetic mRNA into Cells
8. Electroporation of Alphavirus RNA
Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System
Christina L. Gardner, Derek W. Trobaugh,
Kate D. Ryman, and William B. Klimstra
9. GMP-Grade mRNA Electroporation of
Dendritic Cells for Clinical Use
Judith Derdelinckx, Zwi N. Berneman, and Nathalie
Cools
10. Large Scale mRNA Transfection of
Dendritic Cells by Electroporation in Continuous Flow Systems
David Selmeczi, Thomas Steen Hansen,
Özcan Met, Inge Marie Svane, and Niels B. Larsen
11. FLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines
Sebastian Kreiter, Mustafa Diken,
Abderraouf Selmi, Jutta Petschenka, Özlem Türeci, and Ugur Sahin
12. Transfecting Human Monocytes with
RNA
Jens Dannull and Smita K. Nair
13. In Vitro Synthesis, Delivery and
Bioavailability of Exogenous mRNA in Gene Transfer Mediated by PiggyBac Transposition
Solenne Bire, Nicole Ishac, and Florence
Rouleux-Bonnin
14. Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage
Gábor Boros, Katalin Karikó, Hiromi
Muramatsu, Edit Miko, Eszter Emri, Csaba Hegedus, Gabriella Emri, and Éva
Remenyik
Part
IV Alteration of Cellular Function with Synthetic mRNA
15. Delivery of Synthetic mRNA Encoding
FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy
Gayathri R. Devi and Sritama Nath
16. Immune Monitoring using mRNA-Transfected
Dendritic Cells
Troels Holz Borch, Inge Marie Svane, and Özcan
Met
17. Transfection of Tumor-Infiltrating
T Cells with mRNA Encoding CXCR2
Manja Idorn, Per thor Straten, Inge Marie
Svane, and Özcan Met
18. mRNA Electroporation of Dendritic
Cells with WT1, Survivin and TriMix (A Mixture of caTLR4, CD40L, and CD70)
An Coosemans, Sandra Tuyaerts, Kim
Morias, Jurgen Corthals, Carlo Heirman, Kris Thielemans, Stefaan W. Van Gool,
Ignace Vergote, and Frédéric Amant
19. Redirecting T Cell Specificity
Using T Cell Receptor Messenger RNA Electroporation
Sarene Koh, Noriko Shimasaki, and Antonio
Bertoletti
20. Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA
Azita Josefine Mahiny and Katalin Karikó
21. Genetic Modification of Human
Pancreatic Progenitor Cells through Modified mRNA
Song Lu, Christie C. Chow, Junwei Zhou,
Po Sing Leung, Stephen K. Tsui, and Kathy O. Lui