(PDF) Immunological Tolerance – Methods and Protocols by Ashleigh S. Boyd

This is the PDF eBook version for Immunological Tolerance – Methods and Protocols by Ashleigh S. Boyd

Table of Contents

PART I: Isolation of Stem Cells and Their Progeny for Immunological Tolerance Applications
1. Isolation and Characterisation of Human Adipose-derived Stem Cells
Wilson, A., Chee, M.J., Butler P., and Boyd, A.S.
2. Isolation of Murine Hematopoietic Stem Cells
Juan Menendez-Gonzalez, Lubaid Saleh, Gui Jie Feng and Neil P. Rodrigues
3. Generation of Antigen-specific T cells from Human Induced Pluripotent Stem Cells Toshinobu Nishimura and Hiromitsu Nakauchi
PART II: Isolation of Regulatory Lymphocyte Populations
4. Isolation of Human Regulatory T Lymphocytes by Fluorescence-activated Cell Sorting
Kate Milward, Joanna Hester, and Kathryn J Wood
5. Identification and isolation of Regulatory B cells in Mouse and Human
Madhvi Menon, Elizabeth C. Rosser, and Claudia Mauri
6. An innovative Standard Operation Procedure for Isolating GMP-grade CD4 CD25 T Cells from Non-mobilized Leukapheresis
W. Zhang, S. M. Watt, and D. J. Roberts
PART III: Mechanisms and Induction of Immunological Tolerance
7. Measuring CTLA-4-dependent Suppressive Function in Regulatory T Cells
Tie Zheng Hou, Omar S Qureshi, and David M. Sansom
8. Alloanergization Method for Inducing Allospecific Hyporesponsiveness in PBMC Exposed to Allostimulation in vitro in the Context of Costimulatory Molecule Blockade
Christine M. Barbon, Kenneth J. Janec Rowan H. Kelner, James E. Norton, and Eva C. Guinan
9. In Vitro Suppression of CD4 T Cell Responses by Murine and Human Myeloid-derived Suppressor Cells
Katerina Vlachou and Panayotis Verginis
10. Disassembling and Re-aggregating the Thymus: The Plus and Minus of Current Assays
Elia Piccinini and Paola Bonfanti
11. Isolation of Highly Viable Thymic Epithelial Cells for use in In Vitro and In Vivo Experiments
Mi-Jeong Kim and Thomas Serwold
PART IV: Transplantation Tolerance
12. Immune Tolerance and Rejection in Organ Transplantation
Jessica Stolp, Masaaki Zaitsu, and Kathryn J Wood
13. Method of Generating Tolerogenic Maturation-resistant Dendritic Cells and Testing for their Immune-regulatory Functions In Vivo in the Context of Transplantation
Sherrie J. Divito and Adrian E. Morelli
PART V: The Breakdown of Immunological Tolerance in Disease
14. Luminex Coupled-EliFACS: A Multiparametric Method to Enumerate and Functionally Characterize Autoreactive T Cells in Human Peripheral Blood
HuiJuan Lv, Khadir Raddassi, and Myra A Lipes
15. Clinical and Functional Evaluation of Ocular Inflammatory Disease Using the Model of Experimental Autoimmune Uveitis Jun Chen, and Rachel R. Caspi
16. Methods of Isolation and Analysis of TREG Immune Infiltrates from Injured and Dystrophic Skeletal Muscle
Burzyn Dalia, Wagers Amy, Mathis Diane, and Cerletti Massimiliano