(PDF) Gene Therapy of Solid Cancers – Methods and Protocols by Walther

This is the PDF eBook version for Gene Therapy of Solid Cancers – Methods and Protocols by Wolfgang Walther, Ulrike Stein

Table of Contents

Part I: Immunogene Therapy

1. Aptamer Targeting the ERBB2 Receptor Tyrosine Kinase for Applications in Tumor Therapy

Georg Mahlknecht, Michael Sela, and Yosef Yarden

2. Gene Gun Her2/neu DNA Vaccination: Evaluation Of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model

Tam Nguyen-Hoai, Antonio Pezzutto, and Jörg Westermann

3. MIDGE Technology for the Production of a Fourfold Gene-Modified, Allogenic Cell-Based Vaccine For Cancer Therapy

Manuel Schmidt, Barbara Volz, Patrick Großmann, Kerstin Heinrich, and Burghardt Wittig

Part II: Suicide Gene Therapy

4. Evaluation of Bystander Cell Killing Effects in Suicide Gene Therapy of Cancer: Engineered Thymidylate Kinase (TMPK)/AZT Enzyme-Prodrug Axis

Takeya Sato, Anton Neschadim, Ryo Nakagawa, Teruyuki Yanagisawa, and Jeffrey A. Medin

5. Oncoleaking: Use of the Pore-forming Clostridium perfringens Enterotoxin (CPE) for Suicide Gene Therapy

Jessica Pahle, Jutta Aumann, Dennis Kobelt, and Wolfgang Walther

6. iCaspase 9 Suicide Gene System

Xiaoou Zhou, Antonio Di Stasi, and Malcolm K. Brenner

Part III: Gene Replacement Therapies

7. p53-encoding pDNA Purification by Affinity-Chromatography for Cancer Therapy

Ângela Sousa, João A. Queiroz, and Fani Sousa

8. A qRT-PCR Method for Determining the Biodistribution Profile of a miR-34a Mimic

Kevin Kelnar and Andreas G. Bader

Part IV: Gene Suppression and Signaling Modulation Therapy

9. Design and Selection of Antisense Oligonucleotides Targeting Transforming Growth Factor Beta (TGF-b) Isoform mRNAs for the Treatment of Solid Tumors

Frank Jaschinski, Hanna Korhonen, and Michel Janicot

10. RNAInterference for Antimetastatic Therapy

Mathias Dahlmann and Ulrike Stein

11. STAT3 Decoy ODN Therapy for Cancer

Xiaoxia Sun and Jian Zhang

Part V: Oncolytic Virotherapy and Bacterial Vectors for Cancer Treatment

12. Oncolytic Viral Therapy using Reovirus

Chandini Thirukkumaran and Don G. Morris

13. Use of GLV-1h68 for Vaccinia Virotherapy and Monitoring

Alexa Frentzen, Ulrike Geissinger, Desislava Tsoneva, and Jochen Stritzker

14. Back To The Future: Are Tumor-Targeting Bacteria The Next Generation Cancer Therapy?

Robert M. Hoffman

Part IV: Clinical Application of Cancer Gene Therapy

15. Ethics of Cancer Gene Transfer Clinical Research

Jonathan Kimmelman

16. Planning an Academic Clinical Trial

Kim M. Champion and Gemma R. Jones

17. Production of Plasmid DNA as Pharmaceutical
Marco Schmeer and Martin Schleef

18. Minicircle – Next Generation DNA Vectors for Vaccination

Schleef, M., Schirmbeck, R., Michael Reiser2, M.-L. Michel, and Schmeer, M.

VII: Prools of Clinical Cancer Gene Therapy

19. A Phase 2, Open-Label, Randomized Study of Pexa-Vec (JX-594) Administered by Intratumoral Injection in Patients with Unresectable Primary Hepaellular Carcinoma

Caroline J. Breitbach, Anne Moon, James Burke, Tae-Ho Hwang, and David H. Kirn

20. Antiangiogenic Metargidin Peptide (AMEP) Gene Therapy in Disseminated Melanoma
Iben Spanggaard and Julie Gehl

21. Clinical Evaluation of ErbB-targeted CAR T-cells, Following Intra-Cavity Delivery in Patients with ErbB-Expressing Solid Tumors
Sophie Papa, May van Schalkwyk, and John Maher