(PDF) Epstein Barr Virus – Methods and Protocols by Janos Minarovits

This is the PDF eBook version for Epstein Barr Virus – Methods and Protocols by Janos Minarovits, Hans Helmut Niller

Table of Contents

1. Current Trends and Alternative Scenarios in EBV Research
Janos Minarovits and Hans-Helmut Niller

2. Epstein-Barr Virus: Clinical Diagnostics
Hans-Helmut Niller and Georg Bauer

3. Establishment of EBV-Infected Lymphoblastoid Cell Lines
Noemi Nagy

4. Generation and Infection of Organotypic Cultures with Epstein-Barr Virus
Rachel M. Temple, Craig Meyers, and Clare E. Sample

5. Affinity Purification-Mass Spectroscopy Methods for Identifying Epstein-Barr Virus-Host Interactions
Anna A. Georges and Lori Frappier

6. The Use of 3D Telomere FISH for the Characterization of the Nuclear Architecture in EBV-Positive Hodgkin’s Lymphoma
Hans Knecht and Sabine Mai
7. Analysis of EBV Transcription Using High-Throughput RNA Sequencing
Tina O’Grady, Melody Baddoo, and Erik K. Flemington
8. Analysis of Viral Promoter Usage in EBV-Infected Cell Lines: A Comparison of qPCR following Conventional RNA Isolation and Nuclear Run-On Assay
Kalman Szenthe and Ferenc Banati
9. Analysis of Viral and Cellular MicroRNAs in EBV Infected Cells
Rebecca L. Skalsky
10. Isolation and Characterization of Exosomes Released by EBV Immortalized Cells
Gulfaraz Khan and Waqar Ahmed
11. Functional Analysis of Exosomes Derived from EBV-Infected Cells
Gulfaraz Khan and Pretty S. Philip
12. Terminal Repeat Analysis of EBV Genomes
Ferenc Banati, Anita Koroknai, Kalman Szenthe
13. Characterization of EBV Promoters and Coding Regions by Sequencing PCR-Amplified DNA Fragments
Kalman Szenthe and Ferenc Banati
14. The Use of Chromatin Precipitation Coupled to DNA Sequencing (ChIP-Seq) for the Analysis of Zta Binding to the Human and EBV Genome
Anja Godfrey, Sharada Ramasubramanyan, and Alison J. Sinclair
15. Analysis of Viral Epigenotypes Using Bisulfite Sequencing: A Detailed Protocol for the Crucial Bisulfite Modification and PCR Amplification Steps
Daniel Salamon