(PDF) RNA Vaccines – Methods and Protocols by Thomas Kramps

This is the PDF eBook version for RNA Vaccines – Methods and Protocols by Thomas Kramps, Knut Elbers

Table of Contents

1. Introduction to RNA Vaccines
Thomas Kramps and Knut Elbers
Part I: Self-replicating RNA Vectors
2. Self-replicating RNA
Birke A. Tews and Gregor Meyers
3. Self-replicating RNA Vaccine Delivery to Dendritic Cells
Thomas Démoulins, Pavlos C. Englezou, Panagiota Milona, Nicolas Ruggli, Nicola Tirelli, Chantal Pichon, Cédric Sapet, Thomas Ebensen, Carlos A. Guzmán, and Kenneth C. McCullough
4. Plant Expression of Trans-encapsidated Viral Nanoparticle Vaccines with Animal RNA Replicons
Yiyang Zhou, Alison A. McCormick, and Christopher M. Kearney
Part II: Non-replicating mRNA Vectors
5. RNActive® Technology: Generation and Testing of Stable and Immunogenic mRNA Vaccines
Susanne Rauch, Johannes Lutz, Aleksandra Kowalczyk, Thomas Schlake, and Regina Heidenreich
6. Nucleoside Modified mRNA Vaccines for Infectious Diseases
Norbert Pardi and Drew Weissman
7. Generation and Evaluation of Prophylactic mRNA Vaccines against Allergy
Richard Weiss, Sandra Scheiblhofer, and Josef Thalhamer
Part III: Adjuvantation and Delivery
8. Measuring the Adjuvant Activity of RNA Vaccines
Norbert Pardi and Drew Weissman
9. Generation of Immunostimulating 130 nm Protamine-RNA Nanoparticles
Marina Tusup and Steve Pascolo
10. Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins
Kerstin F. Gerer, Stefanie Hoyer, Jan Dörrie, and Niels Schaft
11. Adjuvant-Enhanced mRNA Vaccines
Lukasz Bialkowski, Kevin Van der Jeught, Dries Renmans, Alexia van Weijnen, Carlo Heirman, Marleen Keyaerts, Karine Breckpot, and Kris Thielemans
12. Enhanced Delivery of DNA or RNA Vaccines by Electroporation
Kate E. Broderick and Laurent M. Humeau
Part IV: Preclinical and Clinical Development
13. The European Regulatory Environment of RNA-Based Vaccines
Thomas Hinz, Kajo Kallen, Cedrik M. Britten, Bruno Flamion, Ulrich Granzer, Axel Hoos, Christoph Huber, Samir Khleif, Sebastian Kreiter, Hans-Georg Rammensee, Ugur Sahin, Harpreet Singh-Jasuja, Özlem Türeci, and Ulrich Kalinke
14. Discovery and Subtyping of Neo-Epitope Specific T-cell Responses for Cancer Immunotherapy: Addressing the Mutanome
Mustafa Diken, Mathias Vormehr, Christian Grunwitz, Sebastian Kreiter, Özlem Türeci, and Ugur Sahin
15. Considerations for Producing mRNA Vaccines for Clinical Trials
Andreas Schmid
16. Nonclinical Safety Testing of RNA Vaccines
Gundel Hager
17. Immunotherapy of Uveal Melanoma: Vaccination Against Cancer
Mirko Kummer and Beatrice Schuler-Thurner