This is the PDF eBook version for Developmental Toxicology – Methods and Protocols 2nd Edition by Jason M. Hansen, Louise M. Winn
Table of Contents
1. In Vitro Models in Developmental Toxicology
Louise M. Winn
2. Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments
Jason M. Hansen and Ted B. Piorczynski
3. Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology
Charles A. Easley
4. Neural Crest Cell Models of Development and Toxicity: Cytotoxicity Assay Using Human Pluripotent Stem Cell-Derived Cranial Neural Crest Cell Model
Mika Suga and Miho K. Furue
5. Micromass Methods for the Evaluation of Developmental Toxicants
Omar J. Mohammed and Margaret K. Pratten
6. The Murine Limb Bud in Culture as an In Vitro Teratogenicity Test System
France-Hélène Paradis, Han Yan, Chunwei Huang, and Barbara F. Hales
7. Embryonic Mid-Facial Palatal Organ Culture Methods in Developmental Toxicology
Barbara D. Abbott
8. Ex Vivo Fetal Whole Ovarian Culture Model: An Essential Tool for Studies in Reproductive Toxicology and Pharmacology
Jone A. Stanley, Joe A. Arosh, Patricia B. Hoyer, and Sakhila K. Banu
9. Hepatotoxicity in Zebrafish Larvae
Kathryn Bambino, Joshua Morrison, and Jaime Chu
10. Drosophila as a Model for Developmental Toxicology: Using and Extending the Drosophotoxicology Model
Joslynn G. Affleck and Virginia K. Walker
11. The Chicken Embryo as a Model in Developmental Toxicology
Michael R. Stark and Micah M. Ross
12. The Xenopus tropicalis Model for Studies of Developmental and Reproductive Toxicity
Cecilia Berg
13. Mouse Whole Embryo Culture
Emily W.Y. Tung and Louise M. Winn
14. Rat Whole Embryo Culture
Craig Harris
15. Rabbit Whole Embryo Culture
Terence R.S. Ozolinš
16. Genome Editing in Zebrafish Using CRISPR-Cas9: Applications for Developmental Toxicology
Brendon K. Warner, Jonathan K. Alder, and Arminda Suli
17. Identifying Toxicant-Interacting Genes Using Forward-Genetic Screening in Zebrafish
Jonathon T. Hill
18. Assessment of Histiotrophic Nutrition Using Fluorescent Probes
Craig Harris
19. Examining Gene Expression Patterns through Whole Mount In Situ Hybridization
Jeffery R. Barrow
20. Confocal Laser Scanning Microscopy of Morphology and Apoptosis in Organogenesis-Stage Mouse Embryos
Robert M. Zucker and John M. Rogers
21. Measurement of the Oxidative DNA Lesion 8-Oxoguanine (8-oxoG) by ELISA or by High-Performance Liquid Chromatography (HPLC) with Electrochemical Detection
Danielle M. Drake, Aaron M. Shapiro, and Peter G. Wells
22. Quantifying Activity for Repair of the DNA Lesion 8-Oxoguanine by Oxoguanine Glycosylase 1 (OGG1) in Mouse Adult and Fetal Brain Nuclear Extracts Using Biotin-Labeled DNA
Shama Bhatia and Peter G. Wells
23. Western Analysis of Breast Cancer 1 Protein (BRCA1)
Danielle M. Drake and Peter G. Wells
24. Regulation and Control of AP-1 Binding Activity in Embryotoxicity
Terence R.S. Ozolinš
25. Characterization of Epigenetic Histone Activation/Repression Marks in Sequences of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIP-qPCR)
Shama Bhatia, Jason Matthews, and Peter G. Wells
26. The Application of High-Resolution Ultrasound for Assessment of Cardiac Structure and Function Associated with Developmental Toxicity
Rebecca Maciver and Terence R.S. Ozolinš
27. Assessment of Gross Fetal Malformations: The Modernized Wilson Technique and Skeletal Staining
Robert E. Seegmiller, Nathan Cook, Korance Goodwin, Timothy Leishman, and Michelle Graf